PDBe 2x0b

X-ray diffraction
4.33Å resolution

Crystal structure of human angiotensinogen complexed with renin

Released:

Function and Biology Details

Reaction catalysed:
Cleavage of Leu-|-Xaa bond in angiotensinogen to generate angiotensin I. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Renin Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 383 amino acids
Theoretical weight: 42.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00797 (Residues: 24-406; Coverage: 100%)
Gene name: REN
Sequence domains:
Angiotensinogen Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 452 amino acids
Theoretical weight: 49.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01019 (Residues: 34-485; Coverage: 100%)
Gene names: AGT, SERPINA8
Sequence domains: Serpin (serine protease inhibitor)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R3
Unit cell:
a: 212.475Å b: 212.475Å c: 474.061Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.31 0.309 0.334
Expression system: Escherichia coli BL21(DE3)