› 2wy3

X-ray diffraction
1.8Å resolution

Structure of the HCMV UL16-MICB complex elucidates select binding of a viral immunoevasin to diverse NKG2D ligands

Released:
DOI: 10.2210/pdb2wy3/pdb
PDB entry 2wy3 coloured by chain, front view.
PDB entry 2wy3 coloured by chain, side view.
PDB entry 2wy3 coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the HCMV UL16-MICB complex elucidates select binding of a viral immunoevasin to diverse NKG2D ligands.
Müller S, Zocher G, Steinle A, Stehle T
PLoS Pathog 6 e1000723 (2010)
PMID: 20090832

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147812 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MHC class I polypeptide-related sequence B Chains: A, C
Molecule details ›
Chains: A, C
Length: 319 amino acids
Theoretical weight: 36.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q29980 (Residues: 24-341; Coverage: 88%)
Gene names: MICB, PERB11.2
Sequence domains:
Structure domains: MHC class I-like antigen recognition-like
Protein UL16 Chains: B, D
Molecule details ›
Chains: B, D
Length: 158 amino acids
Theoretical weight: 17.74 KDa
Source organism: Human herpesvirus 5 strain AD169
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P16757 (Residues: 27-184; Coverage: 78%)
Gene name: UL16
Sequence domains: Viral unique long protein 16
Structure domains: Immunoglobulin-like

Ligands and Environments

3 bound ligands:
Ligand PEU 3 x PEU
Ligand ACT 3 x ACT
Ligand NAG 12 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 58.12Å b: 104.17Å c: 146.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.213
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

Quick links

Citations

10 review citations

The adaptable major histocompatibility complex (MHC) fold: structure and function of nonclassical and MHC class I-like molecules.
Adams et al. (2013)
9 more

5 mentions without citation

Structural basis for recognition of cellular and viral ligands by NK cell receptors.
Li et al. (2014)
4 more