› 2wvv

X-ray diffraction
1.73Å resolution

Crystal structure of an alpha-L-fucosidase GH29 from Bacteroides thetaiotaomicron

Released:
DOI: 10.2210/pdb2wvv/pdb
PDB entry 2wvv coloured by chain, front view.
PDB entry 2wvv coloured by chain, side view.
PDB entry 2wvv coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Analysis of the reaction coordinate of alpha-L-fucosidases: a combined structural and quantum mechanical approach.
Lammerts van Bueren A, Ardèvol A, Fayers-Kerr J, Luo B, Zhang Y, Sollogoub M, Blériot Y, Rovira C, Davies GJ
J Am Chem Soc 132 1804-6 (2010)
PMID: 20092273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183665 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Alpha-L-fucosidase Chains: A, C, D
Molecule details ›
Chains: A, C, D
Length: 450 amino acids
Theoretical weight: 52.08 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8A3I4 (Residues: 35-484; Coverage: 99%)
Gene name: BT_2970
Sequence domains: Alpha-L-fucosidase
Structure domains:
Alpha-L-fucosidase Chain: B
Molecule details ›
Chain: B
Length: 450 amino acids
Theoretical weight: 52.08 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8A3I4 (Residues: 35-484; Coverage: 99%)
Gene name: BT_2970
Sequence domains: Alpha-L-fucosidase
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TRS 4 x TRS
Ligand SO4 4 x SO4
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 55.88Å b: 186.87Å c: 97.86Å
α: 90° β: 94.47° γ: 90°
R-values:
R R work R free
0.194 0.192 0.225
Expression system: Escherichia coli BL21

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Citations

5 review citations

Mucin glycan foraging in the human gut microbiome.
Tailford et al. (2015)
4 more

3 mentions without citation

Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
Romero Victorica et al. (2020)
2 more