PDBe 2wvj

X-ray diffraction
2.2Å resolution

Mutation of Thr163 to Ser in Human Thymidine Kinase Shifts the Specificity from Thymidine towards the Nucleoside Analogue Azidothymidine


Function and Biology Details

Reaction catalysed:
ATP + thymidine = ADP + thymidine 5'-phosphate. 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Thymidine kinase, cytosolic Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 193 amino acids
Theoretical weight: 21.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
  • Canonical: P04183 (Residues: 1-193; Coverage: 83%)
Gene name: TK1
Sequence domains: Thymidine kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 156.902Å b: 123.252Å c: 121.024Å
α: 90° β: 132.98° γ: 90°
R R work R free
0.176 0.173 0.221
Expression system: Escherichia coli BL21