PDBe 2wtz

X-ray diffraction
3Å resolution

MurE ligase of Mycobacterium Tuberculosis


Function and Biology Details

Reaction catalysed:
ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate + meso-2,6- diaminoheptanedioate = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L- alanyl-D-gamma-glutamyl-meso-2,6-diaminoheptanedioate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 535 amino acids
Theoretical weight: 55.45 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P9WJL3 (Residues: 1-535; Coverage: 100%)
Gene names: MTCY270.10, Rv2158c, murE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P1
Unit cell:
a: 76.92Å b: 79.8Å c: 82.92Å
α: 111.09° β: 92.16° γ: 93.98°
R R work R free
0.195 0.193 0.251
Expression system: Escherichia coli BL21(DE3)