PDBe 2wtk

X-ray diffraction
2.65Å resolution

Structure of the heterotrimeric LKB1-STRADalpha-MO25alpha complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Calcium-binding protein 39 Chains: A, D
Molecule details ›
Chains: A, D
Length: 341 amino acids
Theoretical weight: 39.92 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y376 (Residues: 1-341; Coverage: 100%)
Gene names: CAB39, CGI-66, MO25
Sequence domains: Mo25-like
Structure domains: Leucine-rich Repeat Variant
Serine/threonine-protein kinase STK11 Chains: C, F
Molecule details ›
Chains: C, F
Length: 305 amino acids
Theoretical weight: 34.3 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15831 (Residues: 43-347; Coverage: 70%)
Gene names: LKB1, PJS, STK11
Sequence domains: Protein kinase domain
Structure domains:
STE20-related kinase adapter protein alpha Chains: B, E
Molecule details ›
Chains: B, E
Length: 373 amino acids
Theoretical weight: 41.75 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q7RTN6 (Residues: 59-431; Coverage: 87%)
Gene names: LYK5, STRAD, STRADA
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P3121
Unit cell:
a: 118.36Å b: 118.36Å c: 390.012Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.24 0.291
Expression system: Spodoptera frugiperda