PDBe 2wsp

X-ray diffraction
2.65Å resolution

Thermotoga maritima alpha-L-fucosynthase, TmD224G, in complex with alpha-L-Fuc-(1-2)-beta-L-Fuc-N3

Released:

Function and Biology Details

Reaction catalysed:
An alpha-L-fucoside + H(2)O = L-fucose + an alcohol
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-L-fucosidase, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 52.21 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9WYE2 (Residues: 1-449; Coverage: 100%)
Gene name: TM_0306
Sequence domains: Alpha-L-fucosidase
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: R32
Unit cell:
a: 180.249Å b: 180.249Å c: 169.733Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.201 0.241
Expression system: Escherichia coli BL21