› 2wsd

X-ray diffraction
1.6Å resolution

Proximal mutations at the type 1 Cu site of CotA-laccase: I494A mutant

Released:
DOI: 10.2210/pdb2wsd/pdb
PDB entry 2wsd coloured by chain, front view.
PDB entry 2wsd coloured by chain, side view.
PDB entry 2wsd coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Proximal mutations at the type 1 copper site of CotA laccase: spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants.
Durão P, Chen Z, Silva CS, Soares CM, Pereira MM, Todorovic S, Hildebrandt P, Bento I, Lindley PF, Martins LO
Biochem J 412 339-46 (2008)
PMID: 18307408

Function and Biology Details

Reactions catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laccase Chain: A
Molecule details ›
Chain: A
Length: 513 amino acids
Theoretical weight: 58.55 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P07788 (Residues: 1-513; Coverage: 100%)
Gene names: BSU06300, cotA, pig
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU 4 x CU
Ligand OXY 1 x OXY
Ligand EDO 12 x EDO
1 modified residue:
Ligand CSX 1 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P3121
Unit cell:
a: 101.868Å b: 101.868Å c: 137.037Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.177 0.19
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Laccase engineering: from rational design to directed evolution.
Mate et al. (2015)
8 more

3 mentions without citation

Chemical approaches to detect and analyze protein sulfenic acids.
Furdui et al. (2014)
2 more