PDBe 2wpt

X-ray diffraction
1.78Å resolution

The crystal structure of Im2 in complex with colicin E9 DNase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin-E9 Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 15.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09883 (Residues: 450-582; Coverage: 23%)
Gene names: cei, col
Structure domains: Colicin e7 immunity protein. Chain B
Colicin-E2 immunity protein Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.95 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P04482 (Residues: 1-86; Coverage: 100%)
Gene names: ceiB, imm
Sequence domains: Colicin immunity protein / pyocin immunity protein
Structure domains: Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 76.229Å b: 82.897Å c: 89.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.223
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli