› 2wia

X-ray diffraction
2.45Å resolution

Crystal Structures of the N-terminal Intracellular Domain of FeoB from Klebsiella Pneumoniae in Apo Form

Released:
DOI: 10.2210/pdb2wia/pdb
PDB entry 2wia coloured by chain, front view.
PDB entry 2wia coloured by chain, side view.
PDB entry 2wia coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Structural fold, conservation and Fe(II) binding of the intracellular domain of prokaryote FeoB.
Hung KW, Chang YW, Eng ET, Chen JH, Chen YC, Sun YJ, Hsiao CD, Dong G, Spasov KA, Unger VM, Huang TH
J Struct Biol 170 501-12 (2010)
PMID: 20123128

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-108087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferrous iron transport protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 29.13 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A6TF32 (Residues: 1-267; Coverage: 35%)
Gene names: KPN_03779, feoB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P63
Unit cell:
a: 84.204Å b: 84.204Å c: 122.457Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.209 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Bacterial ferrous iron transport: the Feo system.
Lau et al. (2016)
3 more

3 mentions without citation

Ins and Outs: Recent Advancements in Membrane Protein-Mediated Prokaryotic Ferrous Iron Transport.
Brown et al. (2021)
2 more