PDBe 2w83

X-ray diffraction
1.93Å resolution

Crystal structure of the ARF6 GTPase in complex with a specific effector, JIP4

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor 6 Chains: A, B, E
Molecule details ›
Chains: A, B, E
Length: 165 amino acids
Theoretical weight: 18.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62330 (Residues: 13-175; Coverage: 93%)
Gene name: ARF6
Structure domains: P-loop containing nucleotide triphosphate hydrolases
C-Jun-amino-terminal kinase-interacting protein 4 Chains: C, D
Molecule details ›
Chains: C, D
Length: 77 amino acids
Theoretical weight: 8.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60271 (Residues: 406-476; Coverage: 5%)
Gene names: HLC6, HSS, KIAA0516, MAPK8IP4, SPAG9, SYD1
Structure domains: arf6 gtpase in complex with a specific effector, jip4

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P6222
Unit cell:
a: 137.27Å b: 137.27Å c: 165.03Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.214 0.238
Expression system: Escherichia coli