PDBe 2w5f

X-ray diffraction
1.9Å resolution

High resolution crystallographic structure of the Clostridium thermocellum N-terminal endo-1,4-beta-D-xylanase 10B (Xyn10B) CBM22-1- GH10 modules complexed with xylohexaose


Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Endo-1,4-beta-xylanase Y Chains: A, B
Molecule details ›
Chains: A, B
Length: 540 amino acids
Theoretical weight: 60.08 KDa
Source organism: Ruminiclostridium thermocellum
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P51584 (Residues: 32-551; Coverage: 50%)
Gene name: xynY
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3221
Unit cell:
a: 173.178Å b: 173.178Å c: 131.71Å
α: 90° β: 90° γ: 120°
R R work R free
0.166 0.164 0.194
Expression system: Escherichia coli BL21(DE3)