PDBe 2w3x

X-ray diffraction
1.75Å resolution

Crystal structure of a bifunctional hotdog fold thioesterase in enediyne biosynthesis, CalE7

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CalE7 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 147 amino acids
Theoretical weight: 17.15 KDa
Source organism: Micromonospora echinospora
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KNG2 (Residues: 1-147; Coverage: 99%)
Gene name: calE7
Sequence domains: Thioesterase-like superfamily
Structure domains: Thiol Ester Dehydrase; Chain A

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2
Unit cell:
a: 101.95Å b: 73.32Å c: 124.41Å
α: 90° β: 98.69° γ: 90°
R-values:
R R work R free
0.196 0.196 0.235
Expression system: Escherichia coli BL21(DE3)