› 2w2e

X-ray diffraction
1.15Å resolution

1.15 Angstrom crystal structure of P.pastoris aquaporin, Aqy1, in a closed conformation at pH 3.5

Released:
DOI: 10.2210/pdb2w2e/pdb
PDB entry 2w2e coloured by chain, front view.
PDB entry 2w2e coloured by chain, side view.
PDB entry 2w2e coloured by chain, top view.
Source organism: Komagataella pastoris
Primary publication:
Crystal structure of a yeast aquaporin at 1.15 angstrom reveals a novel gating mechanism.
Fischer G, Kosinska-Eriksson U, Aponte-Santamaría C, Palmgren M, Geijer C, Hedfalk K, Hohmann S, de Groot BL, Neutze R, Lindkvist-Petersson K
PLoS Biol 7 e1000130 (2009)
PMID: 19529756

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123393 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aquaporin-1 Chain: A
Molecule details ›
Chain: A
Length: 279 amino acids
Theoretical weight: 29.93 KDa
Source organism: Komagataella pastoris
UniProt:
  • Canonical: F2QVG4 (Residues: 1-279; Coverage: 100%)
Gene names: AQY1, PP7435_Chr3-0430
Sequence domains: Major intrinsic protein
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

2 bound ligands:
Ligand BOG 6 x BOG
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I4
Unit cell:
a: 91.447Å b: 91.447Å c: 80.819Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 not available 0.165

Quick links

Citations

26 review citations

Aquaporins: important but elusive drug targets.
Verkman et al. (2014)
25 more

17 mentions without citation

Tuning microbial hosts for membrane protein production.
Freigassner et al. (2009)
16 more