› 2w1n

X-ray diffraction
1.8Å resolution

cohesin and fibronectin type-III double module construct from the Clostridium perfringens glycoside hydrolase GH84C

Released:
DOI: 10.2210/pdb2w1n/pdb
PDB entry 2w1n coloured by chain, front view.
PDB entry 2w1n coloured by chain, side view.
PDB entry 2w1n coloured by chain, top view.
Source organism: Clostridium perfringens
Primary publication:
Portrait of an enzyme, a complete structural analysis of a multimodular {beta}-N-acetylglucosaminidase from Clostridium perfringens.
Ficko-Blean E, Gregg KJ, Adams JJ, Hehemann JH, Czjzek M, Smith SP, Boraston AB
J Biol Chem 284 9876-84 (2009)
PMID: 19193644

Function and Biology Details

Reaction catalysed: 
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171162 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-GlcNAcase NagJ Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 25.92 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0TR53 (Residues: 765-1001; Coverage: 24%)
Gene names: CPF_1442, nagJ
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 148.245Å b: 48.644Å c: 36.9Å
α: 90° β: 96.19° γ: 90°
R-values:
R R work R free
0.228 0.224 0.294
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Insights into the recognition of the human glycome by microbial carbohydrate-binding modules.
Ficko-Blean et al. (2012)
2 more

2 mentions without citation

Portrait of an enzyme, a complete structural analysis of a multimodular {beta}-N-acetylglucosaminidase from Clostridium perfringens.
Ficko-Blean et al. (2009)
1 more