› 2vx8

X-ray diffraction
2.2Å resolution

Vamp7 longin domain Hrb peptide complex

Released:
DOI: 10.2210/pdb2vx8/pdb
PDB entry 2vx8 coloured by chain, front view.
PDB entry 2vx8 coloured by chain, side view.
PDB entry 2vx8 coloured by chain, top view.
Source organisms:
Primary publication:
Molecular basis for the sorting of the SNARE VAMP7 into endocytic clathrin-coated vesicles by the ArfGAP Hrb.
Pryor PR, Jackson L, Gray SR, Edeling MA, Thompson A, Sanderson CM, Evans PR, Owen DJ, Luzio JP
Cell 134 817-27 (2008)
PMID: 18775314

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arf-GAP domain and FG repeat-containing protein 1; Vesicle-associated membrane protein 7 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 169 amino acids
Theoretical weight: 18.68 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P52594 (Residues: 136-175; Coverage: 7%)
  • Canonical: P70280 (Residues: 1-120; Coverage: 55%)
Gene names: AGFG1, HRB, RAB, RIP, Sybl1, Vamp7
Sequence domains: Regulated-SNARE-like domain
Structure domains: Longin domain

Ligands and Environments

1 bound ligand:
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21212
Unit cell:
a: 105.514Å b: 115.102Å c: 55.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.28
Expression system: Escherichia coli BL21

Quick links

Citations

35 review citations

Molecular mechanism and physiological functions of clathrin-mediated endocytosis.
McMahon et al. (2011)
34 more

5 mentions without citation

The planar cell polarity effector Fuz is essential for targeted membrane trafficking, ciliogenesis and mouse embryonic development.
Gray et al. (2009)
4 more