› 2vvp

X-ray diffraction
1.65Å resolution

Crystal structure of Mycobacterium tuberculosis ribose-5-phosphate isomerase B in complex with its substrates ribose 5-phosphate and ribulose 5-phosphate

Released:
DOI: 10.2210/pdb2vvp/pdb
PDB entry 2vvp coloured by chain, front view.
PDB entry 2vvp coloured by chain, side view.
PDB entry 2vvp coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
D-ribose-5-phosphate isomerase B from Escherichia coli is also a functional D-allose-6-phosphate isomerase, while the Mycobacterium tuberculosis enzyme is not.
Roos AK, Mariano S, Kowalinski E, Salmon L, Mowbray SL
J Mol Biol 382 667-79 (2008)
PMID: 18640127

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161734 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase B Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 162 amino acids
Theoretical weight: 17.3 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WKD7 (Residues: 1-162; Coverage: 100%)
Gene names: MTV008.21c, Rv2465c, rpiB
Sequence domains: Ribose/Galactose Isomerase
Structure domains: Sugar-phosphate isomerase, RpiB/LacA/LacB

Ligands and Environments

2 bound ligands:
Ligand R52 5 x R52
Ligand 5RP 5 x 5RP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2
Unit cell:
a: 136.832Å b: 103.058Å c: 69.728Å
α: 90° β: 95.51° γ: 90°
R-values:
R R work R free
0.168 0.166 0.187
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Engineering ribose-5-phosphate isomerase B from a central carbon metabolic enzyme to a promising sugar biocatalyst.
Tang et al. (2021)
13 other articles

3 mentions without citation

Data Intensive Genome Level Analysis for Identifying Novel, Non-Toxic Drug Targets for Multi Drug Resistant Mycobacterium tuberculosis.
Kaur et al. (2017)
2 more