PDBe 2vs6

X-ray diffraction
2.4Å resolution

Function and Biology Details

Reaction catalysed:
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-inactivating protein alpha-trichosanthin Chains: A, B
Molecule details ›
Chains: A, B
Length: 248 amino acids
Theoretical weight: 27.1 KDa
Source organism: Trichosanthes kirilowii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09989 (Residues: 24-270; Coverage: 93%)
Sequence domains: Ribosome inactivating protein

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 70.025Å b: 95.84Å c: 101.832Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.236
Expression system: Escherichia coli BL21(DE3)