PDBe 2vl4

X-ray diffraction
1.9Å resolution

Structural and biochemical evidence for a boat-like transition state in beta-mannosidases

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-mannosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 846 amino acids
Theoretical weight: 97.99 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8AAK6 (Residues: 26-864; Coverage: 100%)
Gene name: BT_0458
Sequence domains: Glycosyl hydrolases family 2
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 90.559Å b: 115.038Å c: 98.697Å
α: 90° β: 112.59° γ: 90°
R-values:
R R work R free
0.165 0.162 0.215
Expression system: Escherichia coli BL21