PDBe 2vkz

X-ray diffraction
4Å resolution

Structure of the cerulenin-inhibited fungal fatty acid synthase type I multienzyme complex

Source organism: Saccharomyces cerevisiae

Function and Biology Details

Reactions catalysed:
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl- carrier protein] + H(2)O. 
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl- carrier protein] + NADH. 
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier- protein]. 
Acetyl-CoA + n malonyl-CoA + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO(2) + 2n NADP(+). 
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier- protein]. 
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate. 
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]. 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl- carrier-protein] + NADPH. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fatty acid synthase subunit beta Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 2051 amino acids
Theoretical weight: 228.94 KDa
Source organism: Saccharomyces cerevisiae
  • Canonical: P07149 (Residues: 1-2051; Coverage: 100%)
Gene names: FAS1, YKL182W
Sequence domains:
Fatty acid synthase subunit alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1887 amino acids
Theoretical weight: 207.18 KDa
Source organism: Saccharomyces cerevisiae
  • Canonical: P19097 (Residues: 1-1887; Coverage: 100%)
Gene names: FAS2, P1409, YPL231W
Sequence domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 231.9Å b: 231.9Å c: 756.8Å
α: 90° β: 90° γ: 90°
R R work R free
0.268 0.268 0.268