PDBe 2vkn

X-ray diffraction
2.05Å resolution

YEAST SHO1 SH3 DOMAIN COMPLEXED WITH A PEPTIDE FROM PBS2

Released:
Source organism: Saccharomyces cerevisiae
Entry authors: Kursula P, Kursula I, Song YH, Paraskevopoulos K, Wilmanns M

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
High osmolarity signaling protein SHO1 Chain: A
Molecule details ›
Chain: A
Length: 70 amino acids
Theoretical weight: 8.2 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P40073 (Residues: 298-367; Coverage: 19%)
Gene names: SHO1, SSU81, YER118C
Sequence domains: SH3 domain
Structure domains: SH3 Domains
MAP kinase kinase PBS2 Chain: C
Molecule details ›
Chain: C
Length: 12 amino acids
Theoretical weight: 1.2 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P08018 (Residues: 92-103; Coverage: 2%)
Gene names: HOG4, J0699, PBS2, SFS4, SSK4, YJL128C

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P65
Unit cell:
a: 67.49Å b: 67.49Å c: 33.17Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.168 0.238
Expression systems:
  • Escherichia coli
  • Not provided