PDBe 2vdu

X-ray diffraction
2.4Å resolution

Structure of trm8-trm82, THE YEAST TRNA m7G methylation complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
tRNA (guanine-N(7)-)-methyltransferase Chains: E, F
Molecule details ›
Chains: E, F
Length: 254 amino acids
Theoretical weight: 29.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12009 (Residues: 39-286; Coverage: 87%)
Gene names: D1075, TRM8, YDL201W
Sequence domains: Putative methyltransferase
Structure domains: Vaccinia Virus protein VP39
tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit TRM82 Chains: B, D
Molecule details ›
Chains: B, D
Length: 450 amino acids
Theoretical weight: 51.38 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03774 (Residues: 1-444; Coverage: 100%)
Gene names: TRM82, YD8358.19, YDR165W

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 53.376Å b: 107.676Å c: 127.749Å
α: 90° β: 91.53° γ: 90°
R-values:
R R work R free
0.228 0.228 0.276
Expression system: Escherichia coli