PDBe 2vck

X-ray diffraction
1.8Å resolution

Structure of Phycoerythrobilin Synthase PebS from the Cyanophage P-SSM2 in Complex with the bound Substrate Biliverdin IXa

Released:

Function and Biology Details

Reaction catalysed:
(3Z)-phycoerythrobilin + 2 oxidized ferredoxin = biliverdin IX-alpha + 2 reduced ferredoxin. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phycoerythrobilin synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 233 amino acids
Theoretical weight: 27.62 KDa
Source organism: Prochlorococcus phage P-SSM2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q58MU6 (Residues: 1-233; Coverage: 100%)
Gene names: PSSM2_058, pebS
Sequence domains: Ferredoxin-dependent bilin reductase

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 53.56Å b: 67.57Å c: 81.28Å
α: 92.26° β: 109.07° γ: 106.58°
R-values:
R R work R free
0.187 0.184 0.225
Expression system: Escherichia coli BL21(DE3)