› 2v7w

X-ray diffraction
1.9Å resolution

X-ray crystal structure of 5'-fluorodeoxyadenosine synthase s158g mutant complexed with 5'-fluorodeoxyadenosin

Released:
DOI: 10.2210/pdb2v7w/pdb
PDB entry 2v7w coloured by chain, front view.
PDB entry 2v7w coloured by chain, side view.
PDB entry 2v7w coloured by chain, top view.
Source organism: Streptomyces cattleya
Primary publication:
Mechanism of enzymatic fluorination in Streptomyces cattleya.
Zhu X, Robinson DA, McEwan AR, O'Hagan D, Naismith JH
J Am Chem Soc 129 14597-604 (2007)
PMID: 17985882

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 299 amino acids
Theoretical weight: 32.37 KDa
Source organism: Streptomyces cattleya
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q70GK9 (Residues: 1-299; Coverage: 100%)
Gene name: flA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand 5FD 3 x 5FD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 75.854Å b: 129.256Å c: 184.485Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.221
Expression system: Escherichia coli BL21(DE3)

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Citations

17 review citations

Halogenation strategies in natural product biosynthesis.
Neumann et al. (2008)
16 more

1 mention without citation

Halogenases: a palette of emerging opportunities for synthetic biology-synthetic chemistry and C-H functionalisation.
Crowe et al. (2021)