PDBe 2v5q

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF WILD-TYPE PLK-1 KINASE DOMAIN IN COMPLEX WITH A SELECTIVE DARPIN

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DESIGN ANKYRIN REPEAT PROTEIN Chains: C, D
Molecule details ›
Chains: C, D
Length: 167 amino acids
Theoretical weight: 17.84 KDa
Source organism: unidentified
Expression system: Escherichia coli
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Serine/threonine-protein kinase PLK1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 315 amino acids
Theoretical weight: 35.97 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53350 (Residues: 32-345; Coverage: 52%)
Gene names: PLK, PLK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 62.329Å b: 135.225Å c: 136.824Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.224
Expression systems:
  • Escherichia coli
  • Spodoptera frugiperda