› 2v40

X-ray diffraction
1.9Å resolution

Human Adenylosuccinate synthetase isozyme 2 in complex with GDP

Released:
DOI: 10.2210/pdb2v40/pdb
PDB entry 2v40 coloured by chain, front view.
PDB entry 2v40 coloured by chain, side view.
PDB entry 2v40 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Welin M, Moche M, Arrowsmith C, Berglund H, Busam R, Collins R, Dahlgren LG, Edwards A, Flodin S, Flores A, Graslund S, Hammarstrom M, Hallberg BM, Holmberg-Schiavone L, Johansson I, Kallas A, Karlberg T, Kotenyova T, Lehtio L, Nyman T, Ogg D, Persson C, Sagemark J, Stenmark P, Sundstrom M, Thorsell AG, Tresaugues L, van den Berg S, Weigelt J, Nordlund P, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate synthetase isozyme 2 Chain: A
Molecule details ›
Chain: A
Length: 459 amino acids
Theoretical weight: 50.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30520 (Residues: 21-456; Coverage: 96%)
Gene names: ADSS, ADSS2
Sequence domains: Adenylosuccinate synthetase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P6322
Unit cell:
a: 155.41Å b: 155.41Å c: 82.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.198
Expression system: Escherichia coli BL21(DE3)

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