PDBe 2uv8

X-ray diffraction
3.1Å resolution

Crystal structure of yeast fatty acid synthase with stalled acyl carrier protein at 3.1 angstrom resolution


Function and Biology Details

Reactions catalysed:
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl- carrier protein] + H(2)O. 
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl- carrier protein] + NADH. 
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier- protein]. 
Acetyl-CoA + n malonyl-CoA + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO(2) + 2n NADP(+). 
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier- protein]. 
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate. 
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl- [acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]. 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl- carrier-protein] + NADPH. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 12-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fatty acid synthase subunit beta Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 2051 amino acids
Theoretical weight: 228.94 KDa
Source organism: Saccharomyces cerevisiae
  • Canonical: P07149 (Residues: 1-2051; Coverage: 100%)
Gene names: FAS1, YKL182W
Sequence domains:
Fatty acid synthase subunit alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1887 amino acids
Theoretical weight: 207.39 KDa
Source organism: Saccharomyces cerevisiae
  • Canonical: P19097 (Residues: 1-1887; Coverage: 100%)
Gene names: FAS2, P1409, YPL231W
Sequence domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 230.6Å b: 230.6Å c: 784.3Å
α: 90° β: 90° γ: 90°
R R work R free
0.2 0.2 0.25