› 2tbs

X-ray diffraction
1.8Å resolution

COLD-ADAPTION OF ENZYMES: STRUCTURAL COMPARISON BETWEEN SALMON AND BOVINE TRYPSINS

Released:
DOI: 10.2210/pdb2tbs/pdb
PDB entry 2tbs coloured by chain, front view.
PDB entry 2tbs coloured by chain, side view.
PDB entry 2tbs coloured by chain, top view.
Source organism: Salmo salar
Primary publication:
Cold adaption of enzymes: structural comparison between salmon and bovine trypsins.
Smalås AO, Heimstad ES, Hordvik A, Willassen NP, Male R
Proteins 20 149-66 (1994)
PMID: 7846025

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trypsin-1 Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 23.78 KDa
Source organism: Salmo salar
Expression system: Not provided
UniProt:
  • Canonical: P35031 (Residues: 21-242; Coverage: 98%)
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand BEN 1 x BEN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 61.95Å b: 84.33Å c: 39.11Å
α: 90° β: 90° γ: 90°
Expression system: Not provided

Quick links

Citations

9 review citations

Psychrophilic enzymes: hot topics in cold adaptation.
Feller et al. (2003)
8 more

7 mentions without citation

Psychrophilic enzymes: from folding to function and biotechnology.
Feller G. (2013)
6 more