› 2rif

X-ray diffraction
2.35Å resolution

CBS domain protein PAE2072 from Pyrobaculum aerophilum complexed with AMP

Released:
DOI: 10.2210/pdb2rif/pdb
PDB entry 2rif coloured by chain, front view.
PDB entry 2rif coloured by chain, side view.
PDB entry 2rif coloured by chain, top view.
Source organism: Pyrobaculum aerophilum str. IM2
Primary publication:
Structures and functional implications of an AMP-binding cystathionine beta-synthase domain protein from a hyperthermophilic archaeon.
King NP, Lee TM, Sawaya MR, Cascio D, Yeates TO
J Mol Biol 380 181-92 (2008)
PMID: 18513746

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CBS domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 141 amino acids
Theoretical weight: 15.31 KDa
Source organism: Pyrobaculum aerophilum str. IM2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8ZVX8 (Residues: 2-138; Coverage: 99%)
Gene name: PAE2072
Sequence domains: CBS domain
Structure domains: CBS-domain

Ligands and Environments

2 bound ligands:
Ligand CS 11 x CS
Ligand AMP 8 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41212
Unit cell:
a: 55.726Å b: 55.726Å c: 336.351Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.239 0.294
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

CBS domains: Ligand binding sites and conformational variability.
Ereño-Orbea et al. (2013)
17 other articles

5 mentions without citation

Structures and functional implications of an AMP-binding cystathionine beta-synthase domain protein from a hyperthermophilic archaeon.
King et al. (2008)
4 more