PDBe 2r9m

X-ray diffraction
1.97Å resolution

Cathepsin S complexed with Compound 15

Released:
Source organism: Homo sapiens
Entry authors: Ward YD, Emmanuel MJ, Thomson DS, Liu W, Bekkali Y, Frye LL, Girardot M, Morwick T, Young ERR, Zindell R, Hrapchak M, DeTuri M, White A, Crane KM, White DM, Wang Y, Hao M-H, Grygon CA, Labadia ME, Wildeson J, Freeman D, Nelson R, Capolino A, Peterson JD, Raymond EL, Brown ML, Spero DM

Function and Biology Details

Reaction catalysed:
Similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cathepsin S Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 24.7 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P25774 (Residues: 115-331; Coverage: 69%)
Gene name: CTSS
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P4122
Unit cell:
a: 85.273Å b: 85.273Å c: 150.331Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.175 0.208
Expression system: Escherichia coli