PDBe 2r9k

X-ray diffraction
2.7Å resolution

Crystal Structure of Misteltoe Lectin I in Complex with Phloretamide

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Beta-galactoside-specific lectin 1 chain A isoform 1 Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 27.82 KDa
Source organism: Viscum album
Expression system: Not provided
UniProt:
  • Canonical: P81446 (Residues: 34-287; Coverage: 48%)
Sequence domains: Ribosome inactivating protein
Structure domains:
Beta-galactoside-specific lectin 1 chain B Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 28.57 KDa
Source organism: Viscum album
Expression system: Not provided
UniProt:
  • Canonical: P81446 (Residues: 302-564; Coverage: 50%)
Sequence domains: Ricin-type beta-trefoil lectin domain
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P6522
Unit cell:
a: 107.059Å b: 107.059Å c: 312.375Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.226 0.267
Expression system: Not provided