PDBe 2r6c

X-ray diffraction
4Å resolution

Crystal Form BH2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 18-mer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Replicative DNA helicase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 454 amino acids
Theoretical weight: 50.7 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X4C9 (Residues: 1-454; Coverage: 100%)
Gene name: dnaB
Sequence domains:
Structure domains:
DNA primase Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 143 amino acids
Theoretical weight: 16.8 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X4D0 (Residues: 455-597; Coverage: 24%)
Gene name: dnaG
Structure domains: DNAb Helicase; Chain A

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3121
Unit cell:
a: 228.775Å b: 228.775Å c: 192.984Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.321 0.32 0.344
Expression system: Escherichia coli BL21(DE3)