PDBe 2r5z

X-ray diffraction
2.6Å resolution

Structure of Scr/Exd complex bound to a DNA sequence derived from the fkh gene

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeobox protein extradenticle Chain: B
Molecule details ›
Chain: B
Length: 63 amino acids
Theoretical weight: 7.55 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P40427 (Residues: 238-300; Coverage: 17%)
Gene names: CG8933, exd
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
Homeotic protein Sex combs reduced Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.97 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P09077 (Residues: 298-384; Coverage: 21%)
Gene names: CG1030, Scr
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
DNA (5'-D(*DAP*DCP*DTP*DCP*DTP*DAP*DAP*DGP*DAP*DTP*DTP*DAP*DAP*DTP*DCP*DGP*DGP*DCP*DTP*DG)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
DNA (5'-D(*DTP*DCP*DAP*DGP*DCP*DCP*DGP*DAP*DTP*DTP*DAP*DAP*DTP*DCP*DTP*DTP*DAP*DGP*DAP*DG)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.13 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 88.84Å b: 92.6Å c: 78.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.303
Expression system: Escherichia coli