PDBe 2r5y

X-ray diffraction
2.6Å resolution

Structure of Scr/Exd complex bound to a consensus Hox-Exd site

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeobox protein extradenticle Chain: B
Molecule details ›
Chain: B
Length: 63 amino acids
Theoretical weight: 7.55 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P40427 (Residues: 238-300; Coverage: 17%)
Gene names: CG8933, exd
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
Homeotic protein Sex combs reduced Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.97 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: P09077 (Residues: 298-384; Coverage: 21%)
Gene names: CG1030, Scr
Sequence domains: Homeobox domain
Structure domains: Homeodomain-like
DNA (5'-D(*DAP*DCP*DTP*DCP*DTP*DAP*DTP*DGP*DAP*DTP*DTP*DTP*DAP*DTP*DGP*DGP*DGP*DCP*DTP*DG)-3') Chain: C
Molecule details ›
Chain: C
Length: 20 nucleotides
Theoretical weight: 6.15 KDa
DNA (5'-D(*DTP*DCP*DAP*DGP*DCP*DCP*DCP*DAP*DTP*DAP*DAP*DAP*DTP*DCP*DAP*DTP*DAP*DGP*DAP*DG)-3') Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.11 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P43212
Unit cell:
a: 65.31Å b: 65.31Å c: 200.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.299
Expression system: Escherichia coli