PDBe 2r40

X-ray diffraction
2.4Å resolution

Crystal structure of 20E bound EcR/USP

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gene regulation protein Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.95 KDa
Source organism: Heliothis virescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7SIF6 (Residues: 3-264; Coverage: 99%)
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Ecdysone receptor Chain: D
Molecule details ›
Chain: D
Length: 266 amino acids
Theoretical weight: 30.37 KDa
Source organism: Heliothis virescens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O18473 (Residues: 305-342, 343-550; Coverage: 43%)
Gene names: EcR, NR1H1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 58.001Å b: 58.001Å c: 303.592Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.224 0.282
Expression system: Escherichia coli BL21(DE3)