PDBe 2r1t

X-ray diffraction
1.7Å resolution

dopamine quinone conjugation to DJ-1

Released:
Source organism: Homo sapiens
Entry authors: Zhongtao Z, Yue F

Function and Biology Details

Reaction catalysed:
(1) An N(omega)-(1-hydroxy-2-oxopropyl)-[protein]-L-arginine + H(2)O = a [protein]-L-arginine + (R)-lactate. (2) An N(6)-(1-hydroxy-2-oxopropyl)-[protein]-L-lysine + H(2)O = a [protein]-L-lysine + (R)-lactate. (3) An S-(1-hydroxy-2-oxopropyl)-[protein]-L-cysteine + H(2)O = a [protein]-L-cysteine + (R)-lactate. 

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein/nucleic acid deglycase DJ-1 Chain: A
Molecule details ›
Chain: A
Length: 187 amino acids
Theoretical weight: 19.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99497 (Residues: 2-188; Coverage: 99%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold
Protein/nucleic acid deglycase DJ-1 Chain: B
Molecule details ›
Chain: B
Length: 187 amino acids
Theoretical weight: 19.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99497 (Residues: 2-188; Coverage: 99%)
Gene name: PARK7
Sequence domains: DJ-1/PfpI family
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P31
Unit cell:
a: 75.468Å b: 75.468Å c: 75.504Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.208 0.208 0.236
Expression system: Escherichia coli