PDBe 2r17

X-ray diffraction
2.8Å resolution

Functional architecture of the retromer cargo-recognition complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vacuolar protein sorting-associated protein 29 Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBQ0 (Residues: 1-182; Coverage: 100%)
Gene names: DC15, DC7, MDS007, VPS29
Sequence domains: Calcineurin-like phosphoesterase superfamily domain
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Vacuolar protein sorting-associated protein 35 Chains: C, D
Molecule details ›
Chains: C, D
Length: 298 amino acids
Theoretical weight: 34.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96QK1 (Residues: 483-780; Coverage: 37%)
Gene names: MEM3, TCCCTA00141, VPS35

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 66.25Å b: 128.471Å c: 140.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.268
Expression system: Escherichia coli BL21(DE3)