PDBe 2qzj

X-ray diffraction
2.89Å resolution

Crystal structure of a two-component response regulator from Clostridium difficile

Released:
Source organism: Clostridioides difficile 630
Entry authors: Sugadev R, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Two-component response regulator Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 136 amino acids
Theoretical weight: 15.72 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q180B0 (Residues: 2-126; Coverage: 55%)
Gene name: CD630_32650
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P41212
Unit cell:
a: 75.044Å b: 75.044Å c: 314.252Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.219 0.285
Expression system: Escherichia coli BL21(DE3)