PDBe 2qwp

X-ray diffraction
1.75Å resolution

Crystal structure of disulfide-bond-crosslinked complex of bovine hsc70 (1-394aa)R171C and bovine Auxilin (810-910aa)D876C in the ADP*Pi form #2

Released:
Source organism: Bos taurus

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heat shock cognate 71 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 43.37 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19120 (Residues: 1-394; Coverage: 61%)
Gene names: HSC70, HSPA8
Structure domains:
Putative tyrosine-protein phosphatase auxilin Chain: B
Molecule details ›
Chain: B
Length: 92 amino acids
Theoretical weight: 10.75 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27974 (Residues: 813-904; Coverage: 10%)
Gene name: DNAJC6
Structure domains: Helix Hairpins

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 38.654Å b: 58.641Å c: 225.558Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.243
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)