PDBe 2qvs

X-ray diffraction
2.5Å resolution

Crystal Structure of Type IIa Holoenzyme of cAMP-dependent Protein Kinase

Released:
Source organism: Mus musculus

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: E
Molecule details ›
Chain: E
Length: 350 amino acids
Theoretical weight: 40.66 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase type II-alpha regulatory subunit Chain: B
Molecule details ›
Chain: B
Length: 310 amino acids
Theoretical weight: 35.3 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12367 (Residues: 92-401; Coverage: 77%)
Gene name: Prkar2a
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 87.9Å b: 92.9Å c: 118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.212 0.251
Expression system: Escherichia coli BL21(DE3)