› 2qtq

X-ray diffraction
1.85Å resolution

Crystal structure of a predicted dna-binding transcriptional regulator (saro_1072) from novosphingobium aromaticivorans dsm at 1.85 A resolution

Released:
DOI: 10.2210/pdb2qtq/pdb
PDB entry 2qtq coloured by chain, front view.
PDB entry 2qtq coloured by chain, side view.
PDB entry 2qtq coloured by chain, top view.
Source organism: Novosphingobium aromaticivorans DSM 12444
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173571 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH tetR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 213 amino acids
Theoretical weight: 24.49 KDa
Source organism: Novosphingobium aromaticivorans DSM 12444
Expression system: Escherichia coli
UniProt:
  • Canonical: Q2G9F6 (Residues: 1-212; Coverage: 100%)
Gene name: Saro_1072
Sequence domains: Tetracyclin repressor-like, C-terminal domain
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

2 bound ligands:
Ligand NO3 3 x NO3
Ligand EDO 24 x EDO
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P212121
Unit cell:
a: 66.465Å b: 98.762Å c: 164.039Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.216
Expression system: Escherichia coli

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