› 2qpn

X-ray diffraction
1.1Å resolution

GES-1 beta-lactamase

Released:
DOI: 10.2210/pdb2qpn/pdb
PDB entry 2qpn coloured by chain, front view.
PDB entry 2qpn coloured by chain, side view.
PDB entry 2qpn coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Structure of GES-1 at atomic resolution: insights into the evolution of carbapenamase activity in the class A extended-spectrum beta-lactamases.
Smith CA, Caccamo M, Kantardjieff KA, Vakulenko S
Acta Crystallogr D Biol Crystallogr 63 982-92 (2007)
PMID: 17704567

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191911 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase GES-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 31.19 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KJY7 (Residues: 1-287; Coverage: 100%)
Gene names: blaGES-1, ges-1
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 42.41Å b: 80.82Å c: 71.44Å
α: 90° β: 101.43° γ: 90°
R-values:
R R work R free
0.135 0.133 0.182
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Carbapenems: past, present, and future.
Papp-Wallace et al. (2011)
4 more

6 mentions without citation

GES-18, a new carbapenem-hydrolyzing GES-Type β-lactamase from Pseudomonas aeruginosa that contains Ile80 and Ser170 residues.
Bebrone et al. (2013)
5 more