› 2qm1

X-ray diffraction
2.02Å resolution

Crystal structure of glucokinase from Enterococcus faecalis

Released:
DOI: 10.2210/pdb2qm1/pdb
PDB entry 2qm1 coloured by chain, front view.
PDB entry 2qm1 coloured by chain, side view.
PDB entry 2qm1 coloured by chain, top view.
Source organism: Enterococcus faecalis V583
Entry authors: Kim Y, Joachimiak G, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + D-glucose = ADP + D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucokinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 326 amino acids
Theoretical weight: 34.73 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q830J4 (Residues: 1-323; Coverage: 100%)
Gene names: EF_2788, glcK
Sequence domains: ROK family
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

5 bound ligands:
Ligand ZN 4 x ZN
Ligand MG 5 x MG
Ligand GOL 6 x GOL
Ligand SO4 2 x SO4
Ligand PEG 1 x PEG
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 62.083Å b: 131.26Å c: 82.763Å
α: 90° β: 92.48° γ: 90°
R-values:
R R work R free
0.19 0.186 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links