PDBe 2qkw

X-ray diffraction
3.2Å resolution

Structural basis for activation of plant immunity by bacterial effector protein AvrPto

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein kinase Chain: B
Molecule details ›
Chain: B
Length: 321 amino acids
Theoretical weight: 36.56 KDa
Source organism: Solanum pimpinellifolium
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q40234 (Residues: 1-321; Coverage: 100%)
Gene name: Pto
Sequence domains: Protein tyrosine kinase
Structure domains:
Avirulence protein Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.28 KDa
Source organism: Pseudomonas syringae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q08242 (Residues: 1-164; Coverage: 100%)
Gene name: avrP
Sequence domains: Central core of the bacterial effector protein AvrPto
Structure domains: Avirulence protein AvrPto

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A, RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 75.465Å b: 94.591Å c: 98.741Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.274 0.271 0.303
Expression system: Escherichia coli BL21