PDBe 2qk4

X-ray diffraction
2.45Å resolution

Human glycinamide ribonucleotide synthetase

Released:

Function and Biology Details

Reactions catalysed:
10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide. 
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. 
ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)- (5-phospho-D-ribosyl)glycinamide. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trifunctional purine biosynthetic protein adenosine-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 48.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P22102 (Residues: 1-430; Coverage: 43%)
Gene names: GART, PGFT, PRGS
Sequence domains:
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 70.28Å b: 79.81Å c: 113.87Å
α: 90° β: 104.07° γ: 90°
R-values:
R R work R free
0.174 0.171 0.221
Expression system: Escherichia coli