PDBe 2qjy

X-ray diffraction
2.4Å resolution

Crystal structure of rhodobacter sphaeroides double mutant with stigmatellin and UQ2

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome b Chains: A, D, G, J, M, P
Molecule details ›
Chains: A, D, G, J, M, P
Length: 445 amino acids
Theoretical weight: 50.16 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02761 (Residues: 1-445; Coverage: 100%)
Gene names: fbcB, petB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1 Chains: B, E, H, K, N, Q
Molecule details ›
Chains: B, E, H, K, N, Q
Length: 269 amino acids
Theoretical weight: 29.37 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02760 (Residues: 23-285; Coverage: 100%)
Gene names: fbcC, petC
Sequence domains: Cytochrome C1 family
Structure domains:
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: C, F, I, L, O, R
Molecule details ›
Chains: C, F, I, L, O, R
Length: 187 amino acids
Theoretical weight: 19.92 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02762 (Residues: 1-187; Coverage: 100%)
Gene names: fbcF, petA
Sequence domains:
Structure domains:

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 351.891Å b: 147.042Å c: 161.312Å
α: 90° β: 104.25° γ: 90°
R-values:
R R work R free
0.237 0.226 0.251
Expression system: Escherichia coli