› 2qju

X-ray diffraction
2.9Å resolution

Crystal Structure of an NSS Homolog with Bound Antidepressant

Released:
DOI: 10.2210/pdb2qju/pdb
PDB entry 2qju coloured by chain, front view.
PDB entry 2qju coloured by chain, side view.
PDB entry 2qju coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
LeuT-desipramine structure reveals how antidepressants block neurotransmitter reuptake.
Zhou Z, Zhen J, Karpowich NK, Goetz RM, Law CJ, Reith ME, Wang DN
Science 317 1390-3 (2007)
PMID: 17690258

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130575 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+):neurotransmitter symporter (Snf family) Chain: A
Molecule details ›
Chain: A
Length: 511 amino acids
Theoretical weight: 57.12 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67854 (Residues: 5-513; Coverage: 99%)
Gene names: aq_2077, snf
Sequence domains: Sodium:neurotransmitter symporter family

Ligands and Environments

5 bound ligands:
Ligand BOG 4 x BOG
Ligand NA 2 x NA
Ligand CL 1 x CL
Ligand LEU 1 x LEU
Ligand DSM 1 x DSM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 88.414Å b: 86.763Å c: 81.187Å
α: 90° β: 95.72° γ: 90°
R-values:
R R work R free
0.199 0.199 0.22
Expression system: Escherichia coli

Quick links

Citations

45 review citations

SLC6 neurotransmitter transporters: structure, function, and regulation.
Kristensen et al. (2011)
44 more

14 mentions without citation

Substrate and drug binding sites in LeuT.
Nyola et al. (2010)
13 more