PDBe 2qjk

X-ray diffraction
3.1Å resolution

Crystal Structure Analysis of mutant rhodobacter sphaeroides bc1 with stigmatellin and antimycin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cytochrome b Chains: A, D, G, J, M, P
Molecule details ›
Chains: A, D, G, J, M, P
Length: 428 amino acids
Theoretical weight: 48.44 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02761 (Residues: 3-430; Coverage: 96%)
Gene names: fbcB, petB
Sequence domains:
Structure domains: Cytochrome Bc1 Complex; Chain C
Cytochrome c1 Chains: B, E, H, K, N, Q
Molecule details ›
Chains: B, E, H, K, N, Q
Length: 256 amino acids
Theoretical weight: 27.79 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02760 (Residues: 23-278; Coverage: 97%)
Gene names: fbcC, petC
Sequence domains: Cytochrome C1 family
Structure domains:
Ubiquinol-cytochrome c reductase iron-sulfur subunit Chains: C, F, I, L, O, R
Molecule details ›
Chains: C, F, I, L, O, R
Length: 179 amino acids
Theoretical weight: 19.06 KDa
Source organism: Rhodobacter sphaeroides
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02762 (Residues: 9-187; Coverage: 96%)
Gene names: fbcF, petA
Sequence domains: Rieske [2Fe-2S] domain
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 352.291Å b: 147.396Å c: 160.762Å
α: 90° β: 104.13° γ: 90°
R-values:
R R work R free
0.239 0.239 0.266
Expression system: Escherichia coli