› 2qjc

X-ray diffraction
2.05Å resolution

Crystal structure of a putative diadenosine tetraphosphatase

Released:
DOI: 10.2210/pdb2qjc/pdb
PDB entry 2qjc coloured by chain, front view.
PDB entry 2qjc coloured by chain, side view.
PDB entry 2qjc coloured by chain, top view.
Source organism: Trypanosoma brucei
Primary publication:
Structural genomics of protein phosphatases.
Almo SC, Bonanno JB, Sauder JM, Emtage S, Dilorenzo TP, Malashkevich V, Wasserman SR, Swaminathan S, Eswaramoorthy S, Agarwal R, Kumaran D, Madegowda M, Ragumani S, Patskovsky Y, Alvarado J, Ramagopal UA, Faber-Barata J, Chance MR, Sali A, Fiser A, Zhang ZY, Lawrence DS, Burley SK
J Struct Funct Genomics 8 121-40 (2007)
PMID: 18058037

Function and Biology Details

Reaction catalysed: 
P(1),P(4)-bis(5'-adenosyl) tetraphosphate + H(2)O = 2 ADP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcineurin-like phosphoesterase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 28.54 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57U41 (Residues: 2-252; Coverage: 100%)
Gene names: Tb08.6H23.310, Tb927.8.8040
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 45.662Å b: 62.207Å c: 81.296Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.247 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Aiding and abetting roles of NOX oxidases in cellular transformation.
Block et al. (2012)
23 more

4 mentions without citation

Structural genomics of protein phosphatases.
Almo et al. (2007)
3 more