› 2qj7

X-ray diffraction
1.05Å resolution

PYP ultra-high resolution of a bacterial photoreceptor

Released:
DOI: 10.2210/pdb2qj7/pdb
PDB entry 2qj7 coloured by chain, front view.
PDB entry 2qj7 coloured by chain, side view.
PDB entry 2qj7 coloured by chain, top view.
Source organism: Halorhodospira halophila
Primary publication:
Picometer-scale conformational heterogeneity separates functional from nonfunctional states of a photoreceptor protein.
Coureux PD, Fan ZP, Stojanoff V, Genick UK
Structure 16 863-72 (2008)
PMID: 18547519

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Photoactive yellow protein Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 13.89 KDa
Source organism: Halorhodospira halophila
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16113 (Residues: 1-125; Coverage: 100%)
Gene name: pyp
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments

1 bound ligand:
Ligand HC4 1 x HC4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P65
Unit cell:
a: 40.621Å b: 40.621Å c: 117.734Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.169 0.168 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Accessing protein conformational ensembles using room-temperature X-ray crystallography.
Fraser et al. (2011)
4 more

2 mentions without citation

Spectroscopic ruler for measuring active-site distortions based on Raman optical activity of a hydrogen out-of-plane vibration.
Haraguchi et al. (2018)
1 more